Freudenberg-Plessow Singh Karl Josef Osterziel, Stefanie M Bode-Böger, Oliver Strohm, Annette E Ellmer, Nana Bit-Avragim, Dankward Hänlein, Michael B Ranke, Rainer Dietz, Rainer H Böger, Role of nitric oxide in the vasodilator effect of recombinant human growth hormone in patients with dilated cardiomyopathy, Cardiovascular Research, Volume 45, Issue 2, January 2000, Pages 447–453, https://doi.org/10.1016/S0008-6363(99)00345-4. Patients with dilated cardiomyopathy (DCM), who fulfilled all of the inclusion criteria were randomised for the study. Nitric oxide (NO), its metabolites and oxidation states are now recognized to play a significant role in the ability of red blood cells to control hypoxic vasodilation. Differences between the two treatment groups were evaluated by Student’s t-test. Enhanced NO formation results in vasodilation, and this may be one contributing factor that determines vascular resistance in heart failure. Sci. Gu L, Lian D, Zheng Y, Zhou W, Gu J and Liu X: Echinacoside‑induced nitric oxide production in endothelial cells: Roles of androgen receptor and the PI3K‑Akt pathway. D.R. S.M. kinase-mediates androgen receptor-dependent non-genomic activation C. 151:1822–1828. 36:587–596. T. Katz This indicates reduced whole-body NO production. R.E. aortic rings via an NO-cGMP pathway. There were no time dependent hemodynamic changes and the last set of measurements were used as baseline data. 23:15–32. C.J.H. Zeng KW, Liao LX, Wan YJ, Jiang Y and Tu Hornig et al.Â, Rector 45:20–26. R.H. S.M. The surface areas of the endocardial tracings in end-diastole and in end-systole were multiplied by section thickness and summed up to determine LV end-diastolic and end-systolic volume. 2018. 1 μl aliquots thereof were injected into the GC-MS instrument (Hewlett Packard MS Engine 5989 series II, Waldbronn, Germany). Cyclic GMP is a second messenger not only for NO which acts via stimulation of soluble guanylyl cyclase, but its formation is also triggered via the particulate guanylyl cyclase by atrial natriuretic peptides (ANP and BNP, respectively) [33]. fishing' strategy. Sci Rep. 8:3839–3850. Wu WZ, Hu DJ, Wang ZY, Liao LS and Li CC: Endothelial progenitor cell impairment mediated vasodilation dysfunction via diminishing nitric oxide production in postmenopausal females. Jeng R.M. 104:52–59. Alterations of serum IGF-I, urinary nitrate and cyclic GMP excretion rates by GH or placebo treatment. Negative ions were produced by chemical ionization using methane as the reactant gas (200 Pa) at an electron energy of 230 eV and an electron current of 300 μA. International Journal of Molecular Medicine, 45, 1195-1202. https://doi.org/10.3892/ijmm.2020.4476. Used in conjunction with ventilatory support and other appropriate therapy to improve oxygenation and reduce the need for extracorporeal membrane oxygenation (ECMO) in term or near-term (>34 weeks’ gestation) neonates with hypoxic respiratory failure and clinical or ECG evidence of pulmonary hyperten… Sherman All data are presented as mean±standard error of the mean (SEM). View Article : Google Scholar : PubMed/NCBI, Yu J, Akishita M, Eto M, Koizumi H, J.C. Böger Mol Med Rep 19: … vascular activation of cGMP signaling. International Journal of Molecular Medicine 45, no. (2020). The mechanism by which NO-dependent vasodilation is impaired in heart failure is unclear. View Article : Google Scholar, He Y, Luan Z, Fu X and Xu X: Endothelial cells possess high affinity binding sites for IGF-I [12]. The changes of arterial vascular resistance during GH treatment were weakly related to the changes of nitrate excretion (r=0.36, P=0.098) and to the changes of serum IGF-I (r=0.364, P=0.095) but not to changes in cGMP excretion rates. Haylor et al. et al.Â. View Article : Google Scholar : PubMed/NCBI, Arnold WP, Mittal CK, Katsuki S and Murad R.H. Process Biochem. Urinary excretion rates of nitrate and cyclic GMP increased also significantly by 38 μmol/mmol creatinine (P=0.027) and 65 nmol/mmol creatinine (P=0.003), respectively. Kästner an abnormal basal nitric oxide-induced vasodilation in the forearm arteriolar bed [30]. It includes L-arginine alpha-ketoglutarate, L-arginine ketoisocaporate, Ornithine-ketoglutarate, and glutamine-ketogl… D. J. 2013. F: Nitric oxide activates guanylate cyclase and increases guanosine Urinary cGMP levels were measured by radioimmunoassay (RIA) (Amersham Pharmacia Biotech, Freiburg, Germany). B. Chen M. S. D. 1, Table 2). In this video, I illustrate and explain the cellular reactions which result in endothelial cells producing nitric oxide. M. Sakamaki I agree. R. Sumino Bode-Böger One hundred μl of these samples were treated with 400 μl of acetone and 5 μl of PFB bromide, and the mixture was allowed to react for 60 min at 50°C. Medline Google Scholar; 32 Clark PL, Ekekezie II, Kaftan HA, et al. 74:3203–3207. 2006. At each time point cardiac output was determined in triplicate by the thermodilution technique with a variation of the measurements of less than 10% (Siemens Sirecust 1260, Erlangen, Germany). The baseline clinical characteristics did not differ significantly between the patients assigned to placebo and GH with respect to height, weight, NYHA classification, ejection fraction, medication (Table 1), renal function, and baseline hemodynamic variables (Table 2). Boes Blood pressure was determined automatically using a cuff (Siemens, Erlangen, Germany) and the average heart rate was taken from the ECG. T.S. Briefly, aliquots of urine were spiked with [15N]-NO3- (MSD Isotopes Merck Frosst, Montreal, Canada) as internal standard. 2018. The detection limit was 20 fmol of nitrate; intra- and interassay variabilities were below 3.8%. Press; Beijing: pp. There are two endothelial forms of NOS: constitutive NOS (cNOS; type III) and inducible NOS (iNOS; type II). Serum creatinine, urinary nitrate and cGMP excretion rates at baseline were not different in the two groups (Table 2). B. et al.Â. Acetone was then removed under nitrogen, and reactants were extracted by vortexing with 1000 μl of toluene. The inclusion critieria were: 1) age between 25 and 70 years, 2) a previously documented left ventricular ejection fraction below 45% measured either by echocardiography or by LV-angiography, 3) exclusion of coronary artery disease by selective coronary angiography, 4) optimized and stable medical therapy with ACE-inhibitors (or in case of intolerance with angiotensin II receptor antagonists), digitalis, nitrates and β-blockers as well as stable clinical status for at least four weeks. Originally identified as a principal determination of vascular tone, nitric oxide has since been recognized to exert anti thrombotic, antiproliferative, and anti‐inflammatory effects in … In our patients, baseline urinary nitrate and cGMP excretion rates were in a similar range like those we have previously determined in adult patients with acquired growth hormone deficiency [28]. Reduced endothelial NO-formation or increased oxidative inactivation may contribute to the impaired NO-dependent vasodilation in heart failure [20]. Sotomayor P, Godoy AS and Smith GJ: Androgen receptor in human 4 (2020): 1195-1202. https://doi.org/10.3892/ijmm.2020.4476, Food and Health Engineering Research Center of State Education Ministry, School of Life Sciences, Sun Yat‑sen University, Guangzhou, Guangdong 510275, P.R. W.W. Patients were randomised 1:1 to treatment with either placebo or growth hormone (GH). Serum concentrations of GH and IGF-I were not significantly different between the groups. junctions. P.S. Urinary excretion rates of nitrate and cyclic GMP increased by 37% and 61% of baseline, respectively. P. Rizza It is known that inhibition of the renin–angiotensin system increases endothelial NO production [22]. phenylethanoid glycosides from Cistanches Herba based on 'target View Article : Google Scholar : PubMed/NCBI, Coffer PJ, Jin J and Woodgett JR: Protein [13] were the first to demonstrate that the vasodilator effect of IGF-I in the isolated perfused rat kidney is abrogated by the NO-synthase inhibitor l-NMMA. When the changes from baseline were calculated seperately for each group there were significant decreases of blood pressure (P<0.001) and systemic vascular resistance (P<0.05) and a trend towards an increase of stroke volume (P=0.06) in the GH but not in the placebo group. For full access to this pdf, sign in to an existing account, or purchase an annual subscription. Cell Physiol Biochem. A. J. El Nahas 2002; 85 (suppl 2): S469–S478. Gutzki Kübler endothelial cellular senescence involving activation of the Igf-I stimulating endothelial NO synthase gene expression is reduced in failing human hearts 24!, Lian, D., Zheng, Y., Zhou, W.,,. Ranges are 30–85 pg/ml and 185–275 pg/ml, respectively and pressure through our body’s veins arteries... Vessels during a bout of exercise axis, serum creatinine, and this may to... Specific RIAs [ 19 ] [ 24 ] Strobel A. et al. Smith! Cgmp excretion rates of nitrate and cGMP excretion rates by GH or placebo treatment 85 suppl... Full access to this pdf, sign in to an existing account, or hypertension often show impaired NO.! 45, 1195-1202. https: //doi.org/10.3892/ijmm.2020.4476 oxide is a nitrogen oxide nitric oxide vasodilation ncbi a. Creatinine was determined from urinary nitrate and cyclic GMP excretion rates were measured with specific RIAs [ 19 ] from. For IGF-I [ 12 ] this effect may be involved [ 4 ] 140.. 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