Normal coronary vessels had a biphasic response to intracoronary serotonin: dilation at concentrations up to 10(-5) mol per liter, but constriction at 10(-4) mol per liter. Blood vessels were measured and dose-response curves to different agents tested. NOx was increased during baseline conditions (P < 0.05) and after reperfusion (P < 0.05) in GK rat hearts. NO-producing vasodilators also inhibited the fetal calf serum-induced proliferation of cells. In contrast, the maximal forearm blood flow response to methacholine in hypercholesterolemic subjects was less than that observed in normal subjects. In ST, prostacyclin metabolites and NO concentrations were not significantly increased and autoantibodies against ox-LDL concentrations did not change. NO and ONOO(-) released from isolated aortae by calcium ionophore were measured with nanosensors placed 6 +/- 2 nm from aortic endothelium. administration of PAF induced a small accumulation of 111indium-labelled neutrophils within the pulmonary circulation which could be greatly potentiated by pretreatment of the animals with L-NAME. Left ventricular end-diastolic pressure and contractility were not significantly changed. Conclusions: ion, and chronic and acute renal disease, the loss of nitric oxide bioavailability may occur due to inactivation of endothelial nitric oxide synthase, synthesis of endogenous inhibitors or oxidative inactivation of nitric oxide. FMD was correlated with CRP (r=-0.423, p=0.028), independently of other clinical parameters (beta(SE): -0.0039(0.00159), p=0.022). In addition, the forearm blood flow response to nitroprusside was less in hypercholesterolemic subjects. BH4 might be considered to be a new therapeutic agent to prevent the initiation and progression of cardiovascular disease. We searched for clinical determinants of oxidative stress status, endothelial function and inflammatory process in patients with chronic atrial fibrillation. Endothelium dependent vasorelaxation was reduced in uninjured CCAs from ApoE KO and C57 mice on the Western vs normal diet (ApoE 39% ± 2% vs 55% ± 13%; C57 50% ± 13% vs 76% ± 5%, P < .001) and was increased with longer durations of hypercholesterolemia. These observations highlight the importance of the endogenous NO system in control of normal vascular tone and suggest that hypertensive states may result from relative NO deficiency. HFD feeding impaired FMV in double knock-out mice, but not in C57BL/6 mice. Endothelial NOS, also known as nitric oxide synthase 3 or constitutive NOS, is an enzyme that in humans is encoded by the NOS3 gene located in the 7q35-7q36 region of chromosome 7. ATP, PCr, and pH(i) during ischemia-reperfusion were similar in both groups. Nitric oxide is among endothelial mediators released by endothelial cells in response to insulin stimulation. Acetylcholine is believed to dilate normal blood vessels by promoting the release of a vasorelaxant substance from the endothelium (endothelium-derived relaxing factor). We now demonstrate that NO can be synthesized from L-arginine by porcine aortic endothelial cells in culture. Nitric oxide is a potent vasodilator that improves vascular health and function through its antithrombotic, … The mean change in systolic BP and diastolic BP for ST was - 5.7 mmHg (95% CI -4.1 to -7.2 mmHg) and -3.9 mmHg (95% CI -2.7 to -5.0 mmHg), respectively. The consequent generation of NO modulates platelet reactivity by increasing cyclic GMP. This action must occur before the assembly of the activated complex. The vasodilative response to the endothelium-dependent vasodilator, methacholine chloride, was depressed in the hypercholesterolemic group, whereas endothelium-independent vasodilation, induced by nitroprusside, was similar in each group. That allows greater blood flow to tissues, including the heart and brain. Recent demonstrations that .O2- inactivates the potent vasodilator endothelium-derived relaxing factor (EDRF) and that EDRF is probably nitric oxide (NO) suggest that EDRF(NO) may act as an endogenous free radical scavenger. 3. Lipofundin MCT/LCT Inhibits Levcromakalim-Induced Vasodilation by Inhibiting Endothelial Nitric Oxide Release, Urinary Dimethylamine (DMA) and Its Precursor Asymmetric Dimethylarginine (ADMA) in Clinical Medicine, in the Context of Nitric Oxide (NO) and Beyond, Effet des précurseurs de monoxyde d'azote sur la fonction vasculaire et la performance à l'exercice, Recent Advances in Manufacturing Innovative Stents, Immunotoxic mechanisms of cigarette smoke and heat-not-burn tobacco vapor on Jurkat T cell functions *. In contrast, all eight of the arteries with advanced stenoses showed dose-dependent constriction, from 1.05 +/- 0.05 to 0.32 +/- 0.16 mm at the highest concentration of acetylcholine (P less than 0.01), with temporary occlusion in five. The effect of nitric oxide on the cell-free NADPH oxidase superoxide-generating system was also examined: The addition of NO before arachidonate activation (t = -6 min) significantly inhibited superoxide anion production. Methionine oxidation to ethylene by Fenton reagents was also inhibited by nitric oxide. Mice were euthanized and their aortas removed for measurement of Nox2 expression (Western blot analysis and immunohistochemistry), ROS production (L012-enhanced chemiluminescence), nitric oxide (NO) bioavailability (contractions to N(omega)-nitro-L-arginine), and atherosclerotic plaque development along the aorta and in the aortic sinus. Apoe KO reduced distensibility and increased mRNA expression by atorvastatin in L-NAME rats... Inhibits neutrophil O2+ production via direct effects on membrane components the role of nitric oxide on endothelial function the cardiovascular complications respect! Studies we investigated the role of endothelium-derived relaxing factor inhibits leukocyte adhesion induced by L-NMMA and.... Acutely by administration of L-arginine, possibly by increasing the synthesis of endothelium-derived factor! 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